Page 102 - Pharma News Letter - Vol2 Issue 1

Chapter V

Formulation Of Lipid Polymer Hybrid Nanoparticles Of The
Phytochemical Fisetin And Its In Vivo Assessment Against
Severe Acute Pancreatitis

Scientific Reports. 2023 Nov; 13(1): 19110.
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Randa Hanie Awadeen , Mariza Fouad Boughdady , Randa A Zaghloul, Wael M

Elsaed, Irhan Ibrahim Abu Hashim, Mahasen Mohamed Meshali

Abstract

Fisetin (FST) is a naturally occurring flavonol that has recently emerged as a bioactive
phytochemical with an impressive array of biological activities. To the author knowledge,
boosting the activity of FST against severe acute pancreatitis (SAP) through a nanostructured
delivery system (Nanophytomedicine) has not been achieved before. Thereupon, FST-loaded
lipid polymer hybrid nanoparticles (FST-loaded LPHNPs) were prepared through conjoined
ultrasonication and double emulsion (w/o/w) techniques. Comprehensive in vitro and in vivo
evaluations were conducted. The optimized nanoparticle formula displayed a high entrapment
efficiency % of 61.76 ± 1.254%, high loading capacity % of 32.18 ± 0.734, low particle size
of 125.39 ± 0.924 nm, low particle size distribution of 0.357 ± 0.012, high zeta potential of +
30.16 ± 1.416 mV, and high mucoadhesive strength of 35.64 ± 0.548%. In addition, it exhibited
a sustained in vitro release pattern of FST. In the in vivo study, oral pre-treatment of FST-
loaded LPHNPs protected against L-arginine induced SAP and multiple organ injuries in rats
compared to both FST alone and plain LPHNPs, as well as the untreated group, proven by both
biochemical studies, that included both amylase and lipase activities, and histochemical studies
of pancreas, liver, kidney and lungs. Therefore, the study could conclude the potential efficacy
of the novel phytopharmaceutical delivery system of FST as a prophylactic regimen for SAP and
consequently, associated multiple organ injuries.

Reference:

https://doi.org/10.1038/s41598-023-46215-8.

102 Faculty of Pharmacy Newsletter