Page 100 - Pharma News Letter - Vol2 Issue 1

Chapter V

Salutary Influence Of Gemifloxacin Mesylate Nanocubosomes
Based-In Situ Ocular Gel As A Novel Approach For The
Management Of Experimental Keratitis Induced By Mrsa

Journal Of Drug Delivery Science And Technology. 2023 Nov; 89: 105012.
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Ghada Ahmed El-Emam, Amira Motawea, Walaa Ibrahim Abd El Hady, Sameh
Saber, Ahmed A.e.mourad, Heba A. Ramadan, Ahmed M. El-Baz

Abstract

In the era of Methicillin-resistant Staphylococcus aureus (MRSA) resulting in the ineffectiveness
of antibiotics is of keen interest to researchers in up-to-date scenarios. The current study aims
to formulate water-soluble drug (Gemifloxacin) loaded nanocubosomes via melt dispersion
emulsification method using glyceryl mono oleate/poloxamer 407 in different ratios. Firstly,
the optimized formula (F6) was evaluated for surface morphology, particle size, polydispersity
index, zeta potential, drug entrapment efficiency%, compatibility studies, and in vitro release.
Secondly, the optimized formulation of nanocubosomes was laden as in situ gel and evaluated
for gelling effect, in vitro release, drug content, stability study, and antimicrobial activity. The
optimized nanocubosomes F6 showed a particle size of less than 200 nm, a zeta potential −26.6
± 1.3 mV, and a high entrapment efficiency of 96.62 ± 2.35%. The developed in situ gel (F6)
shows prolonged drug release and good stability. Our objectives in that study are to investigate
the effectiveness of that novel approach for the management of corneal lesions and inflammation
in a rat model of MRSA keratitis as well as in vitro estimation for their MIC. Results showed
greater potency by having lower MICs than Gemifloxacin solution and significant reductions
in corneal opacity and inflammation score in rats treated with GEM-loaded nanocubosomes
compared to those treated with Gemifloxacin solution. Histopathological analysis demonstrated
a preservation of the normal corneal structure in the GEM-loaded nanocubosomes group. It is
clear from the above-performed studies that the prepared in situ gel would afford a profitable
treatment for keratitis with a lack of irritancy.

Reference:

https://doi.org/10.1016/j.jddst.2023.105012

100 Faculty of Pharmacy Newsletter