Page 97 - Pharma News Letter - Vol2 Issue 1

Research Publications

Comparative Novel Green Reversed-Phase High-Performance
Liquid Chromatography Method ForSimultaneous Determination
Of Ivabradine And Carvedilol In Tablets And Human Plasma
Using Uv And Fluorescence Detectors

Sustainable Chemistry And Pharmacy. 2024; 38: 101476.
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Ethar A. Rashad 1, Samar S. Elsayed 1, Jenny Jeehan M. Nasr 1, 2, Fawzia A. Ibrahim 1

1 Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Mansoura University, Mansoura,
35516, Egypt.

2 Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Mansoura National University,
Gamasa, 7731168, Egypt.

Abstract

A rapid, green, and sensitive dual detection technique method was applied using both UV and
fluorimetric detection to determine ivabradine and carvedilol in their pure form, combined tablet
formulation, and in the spiked human plasma. The method was achieved by using HyperClone™
MOS C8 column (150 × 4.6 mm, with particle size 5 μm), and a mixture composed of
(70:10:20%v/v) ethanol: methanol: 0.02 M potassium phosphate buffer adjusted to pH = 3 b
y using o-phosphoric acid. The dual detection technique was performed at 210 nm in the case
of UV detection, and at 337 nm after excitation at 282 nm in the case of fluorimetric detection
with a flow rate of 1 mL/min. The retention time in the proposed method was found to be 2.13
and 4.72 min for carvedilol and ivabradine respectively (UV detection) and 2.16 and 4.5 min for
carvedilol and ivabradine respectively (fluorescence detection). It was found to be a rectilinear
method (r > 0.999), precise (RSD <2 %), and accurate (recoveries 99.92 and 100.52). The method
was simple, specific, sensitive, and robust. Calibration graphs were found to be rectilinear
from 0.25 to 11.25 μg/mL and 0.50–16 μg/mL for carvedilol and ivabradine for UV detection,
while from 30 to 210 ng/mL and 48–400 ng/mL for carvedilol and ivabradine for fluorimetric
detection. A correlation coefficient (r) was 0.9999 for the two drugs in UV and fluorimetric
detection approaches. Fixed–dose combination tablets containing both medications could be
examined routinely by the validated method without interference from excipients. Moreover,
the fluorimetric detection HPLC technique can be applied to determine the studied drugs in the
human plasma with high accuracy and precision. Greenness of the method was assessed by using
Eco-scale and AGREE metrics, and the method was found to be green.

Reference:

https://doi.org/10.1016/j.scp.2024.101476

Vol. 2 - Issue 1 97