Page 44 - Pharma News Letter Vol1 Issue2 - Converted By Dr. Ahmed Rashed

Chapter VI

Journal of Biochemical and Molecular Toxicology

2023 Mar;37(3):e23266

Fingolimod attenuates ovalbumin-induced airway
inflammation via inhibiting MAPK/ERK signaling in mice

Mirhan N. Makled , Ahmed R. El-Sheakh ,
1 2
1
1 Department of Pharmacology and Toxicology,
Faculty of Pharmacy, Mansoura University,
Mansoura, Egypt.

2 Future Studies and Risks Management &
National Committee of Drugs, Academy
of Scientific Research, Ministry of Higher
Education, ElSayeda Zeinab, Egypt.
Abstract

The current study was designed to investigate
the potential anti-inflammatory and antioxidant
effects of fingolimod against Ovalbumin (Ova)-
induced allergic airway inflammation compared
to dexamethasone. Fingolimod was given (0.5
mg/kg/day, p.o.) for sensitized mice 1 h before
Ova challenge from Days 19 to 24. Fingolimod challenged mice. In conclusion, the current
significantly inhibited Ova-induced elevation study demonstrated the anti-inflammatory and
of inflammatory cells and eosinophils numbers antioxidant effects of fingolimod in allergic
in bronchoalveolar lavage fluid (BALF) and airway inflammation that might be associated
reduced concentrations of immunoglobulin with the downregulation of mitogen activated
E in serum and of sphingosine-1-phosphate, kinases signaling to decrease T helper 2
interleukin (IL)-4, and IL-13 in BALF. cytokine secretion (IL-4 and IL-13) and MCP-
Fingolimod inhibited microvascular leakage 1 expression, along with the inhibition of
and edema as reflected by the decreased lung/ oxidative stress.
body weight index. These findings were
supported by histopathological examination https://doi.org/10.1002/jbt.23266
results showing that fingolimod substantially
decreased perivascular edema and inflammatory
cell infiltration. Fingolimod also attenuated
Ova-induced oxidative stress as evidenced
by decreased malondialdehyde concentration
along with increasing concentrations of reduced
glutathione and superoxide dismutase in lung
tissues. Fingolimod also significantly decreased
monocyte chemoattractant protein-1 (MCP-
1), p-ERK, and p-P38 in lung tissues of Ova-

44 Faculty of Pharmacy Newsletter